Kien Voon KONG
National Taiwan University, Taiwan
Organometallics-based Sers Reporters
Optical imaging is a very important tool in biological discovery, and remains a very important goal as an in vivo imaging modality. There is a demand for the multiplexed detection of disease biomarkers, i.e., detecting the presence and tracking of more than one target, coupled with ultra-high sensitivity, for in vivo applications in animal models and cells. Currently, these are based SERS nanotags, the sensitivity of which depends inherently on the signal strengths of the Raman reporter, most of which are organic compounds with signals that tend to overlap with those of the cellular contents. A class of imaging tags which we have been developing over the last decade or so has been that based on organometallic (OM) compounds, particularly transition metal carbonyl compounds. These compounds show very strong absorption bands in the mid-IR, at around 2000 cm-1, for the CO stretch and are advantageous for cellular imaging as the region is devoid of interference from absorptions by the cellular contents. This presentation will provide a detailed coverage of recent highlights in SERS as a biosensing and bioimaging modality. A brief overview of SERS will be discussed along with the underlying mechanisms for the Raman enhancement. Recent advancements in clinically-relevant OM based SERS-active plasmonic nanomaterials will be detailed in terms of their designs, sensitivity. This will be followed by an assortment of applications in imaging and bio-sensing.